Following adeno-associated virus (AAV)-mediated transduction, cellular RNA preparations can be contaminated with AAV single-stranded DNA. The single-stranded DNA genome of recombinant AAV vectors can ...

The Indian Council of Medical Research (ICMR) has evaluated a new and simplified RT-PCR testing method to detect SARS-Cov-2, which can potentially reduce testing time and cost. The new diagnostic ...

One of the best ways to figure out how far a virus like COVID-19 has spread in a community is to test for it. This step-by-step guide explains how coronavirus swab-the-nose-and-throat tests work, and ...

CSIR-CCMB’s novel ‘Dry Swab’, extraction-free direct RT-PCR testing method, which has reduced the time taken for the COVID test result to be declared, got further validation with another study ...

Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

The modular multiplex RT-PCR method simultaneously detects Shiga Toxin E. coli (STEC), Salmonella spp. and Listeria monocytogenes using a single, overnight enrichment (24 hours). Ready-to-use PCR ...

In this study, an optimized new, simple, low cost method for sampling of airborne viruses using Low Melting Agarose (LMA) plates and a conventional microbial air sampling device has been developed.

Noroviruses (Caliciviridae) are one of the main cause of acute non-bacterial gastroenteritis in humans. Noroviruses can not be propagated in cell cultures. Thus Real Time PCR molecular methods for ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...

Researchers develop a molecular testing-based fast-throughput method for the rapid and early detection of Salmonella ...